BSC 2011 FIU Antibiotic Properties in Essential Oils Through Limitations of E Coli Growth Lab BSC 2011L Lab Report Guidelines General Guidelines The lab re

BSC 2011 FIU Antibiotic Properties in Essential Oils Through Limitations of E Coli Growth Lab BSC 2011L Lab Report Guidelines General Guidelines The lab report must be in APA format: – typed double spaced 12 pt font, Times New Roman 1 inch margins pages numbered on the top right, running header on the top left titled sections untitled hypothesis Quotes are NOT allowed. Everything must be properly paraphrased. No website references as sources. No exceptions. Everything must be properly cited. It is considered plagiarism if it is not. Paper must be in third person, past tense o Note: Never write statements like the following: “My lab report is about…”, “My hypothesis is…”, or any version of this type of statement. For more information on how to cite in APA, you may visit https://owl.purdue.edu/owl/research_and_citation/a… The lab report will be composed of seven sections. Each section will have a heading immediately followed by the text, figures or graphs. The order of the sections is: cover/title page, abstract, introduction, methods, results, discussion and literature cited. Cover Page You must include a cover page with your name, panther ID, BSC2011L, your section, TA name, date, and title of your paper. Create a title that briefly conveys to the reader the purpose of the paper. The title of your report must be informative. Many readers scan journal article titles and the decision whether or not to pursue an article is based on the information in the title. Generally, this information includes primary factor(s) manipulated or studied, outcome of manipulation (the response or effects) and organism studied, if relevant. Abstract The abstract should be a quick but comprehensive summary of the entire report. It serves to help readers determine how relevant the report is to their own interests. This section should only be one paragraph and should include what was done, the reasoning behind it, the results and the conclusions. It must highlight only the most important elements of each major sections of the report (Introduction, Methods, Results, and Discussion). Only the most important aspects of the report should make it to the abstract. This section should be between 200 and 250 words in length and should contain a clear summary of what was demonstrated, how each part of the lab was carried out and how conclusions were reached. It should also be on its own page. Introduction This section contains the basic background information for the lab report. Be sure to comment on what is the significance of this study and its relation to the larger field. Give an example of why the study is significant. After reading the introduction, the reader should have a good idea of the general purpose of the experiment and the importance of the problem being addressed. Here, you will also introduce the purpose of your experiment. You should also be including your hypothesis and predictions in this section – which should be based in the introduction (without saying “My predictions are…” or “My hypothesis is…”). This section should be about 2 pages long and smoothly flow from one topic to the next. All of your background information should be presented here, so the majority of your citations should be found here. You must include 10 sources, 5 of which are primary sources. Remember: do NOT quote anything and make sure you are properly paraphrasing – otherwise, you are PLAGIARIZING. Methods In this section, you must carefully outline the procedures and techniques used in a way that a reader can replicate the experiment based on the information provided here. This section should also include models or equipment used (this should not be written like a laundry list of materials), source of chemicals (if relevant), numbers and types of organisms used, including sex and strain sample sizes, number of times experimental procedure was performed, and other pertinent factors. Keep in mind the you used the methods in the lab manual, so you should be citing the lab manual. Please note: This section must be a NARRATIVE – not bullet points or a numbered list of steps. No credit will be given for a methods section written in a bulleted format. Also, it must be past tense and in 3rd person – you should not be saying “first, I did this…..” and so on. Results In this section, you will be presenting all of the observations and findings from the experiment. It is crucial that the outcomes of your experiments are carefully organized and clearly presented, and you must do so using tables, graphs and pictures wherever necessary. Each figure should have a title or number and brief caption that explains the information being presented by the figure. All graphs and tables should be your own work! Graphs and tables should not be identical to anyone else’s in the lab, including your lab partner’s. Please note: You should NOT be interpreting the results in this section!! This section focuses on what you observed quantitatively and qualitatively throughout the collection of data. Any and all analysis belongs in the discussion section. Discussion • In the discussion section, you should begin this section with restating the purpose and hypothesis of your experiment. This section is where results are interpreted, and conclusions are drawn. The significance and interpretation of the study should be explained in this section as well. You should be referencing figures in the results sections and explaining what these figures mean. Because you restated the hypothesis before interpreting the results, you should be indicating whether or not the results support your hypothesis – keyword: SUPPORT. Remember nothing is EVER proven, so the results either support or refute your original hypotheses/predictions. In addition to explaining the results, you have to indicate the significance of your results. What does this mean? How is it relevant or important? How can this information be applied to the real world? If there were any errors made or any limitations to the experimental design, this should also be included and in context with the interpretation of the results. Please note: If you indicate any errors or limitations, you must present SOLUTIONS to these issues, in the event that this experiment will be replicated. This section should be approximately 2-3 pages long and should be the longest part of your lab report. Literature Cited This section includes the alphabetical listing of all sources cited in your paper. This will primarily include research articles but may include review articles and texts as well. The citations must be written using APA format. You need include at least 10 references, 5 of which must be primary sources. You may include your general biology textbook and lab manual (don’t forget to cite the methods). No website references or any encyclopedia references are allowed. For each source in this section, YOU MUST HAVE IN-TEXT CITATIONS! Any intext citation in the body of the report is put at the end of a sentence and should be done in APA format. General APA citation Guidelines: 1. References should be listed in alphabetical order 2. If a particular reference takes more than one line, then all lines (except the first) should be indented 3. Include ALL authors, never write “et al.,” when writing the full literature cited page 4. Remember to write “and” before the name of the last author, if the article or book has multiple authors. 5. References should not be numbered nor bulleted. 6. Everything counts including but not limited to the placement of period, italics, parentheses, etc. 7. If there are no in-text citations and no works cited section the report will be considered plagiarized. General Rules For the Lab Report: • • • • • If you are not present in lab you cannot turn-in a lab report for that lab. You will turn in your lab report in its entirety. Turning in one or more components or sections of the lab report after the due date and time will make the entire lab report late. This lab report must be your own work (no plagiarism). Content used from references needs to be cited. All text and graphs should be original and not the same as your partners. You cannot resubmit work previously done in another class (per the plagiarism policy) You will need to turn in only an electronic copy. Turnitin will be used to check for plagiarism. 10 points per day will be deducted from your lab report until your instructor has your report through Turnitin. It is your responsibility to turn your lab report on time electronically to your instructor, per their instructions.

Does the cover page have an informative title? Is all information (name, section, etc.) included? 2 Does the abstract reflect the entirety of the paper, including methods, results and limitations? Is the abstract on its own page? Is the abstract length adequate (1 paragraph, 200-250 words)? 7 Is the topic clearly introduced and well researched? Is the purpose of the experiment explained? Is there a hypothesis? Is the introduction at least 2 pages long? 20 Methods Are the procedures correct? Are all steps included? Is this section a past-tense narrative (NOT in bullet points)? 6 Results Are the observations clearly explained? Are there relevant pictures/graphs/tables included, and if so, is there a description under each? Are results analyzed in this section? (they should NOT be). 10 Are the purpose and hypothesis restated? Are the results well discussed? Are real-world applications and significance of findings included? Are limitations and sources of error evaluated, and are solutions provided for these? 25 Is paper in APA format? Is the information clear and concise? Does the paper flow and transition well, with no jumping between topics? 10 Is there proper grammar, spelling and paraphrasing? Are sentences structured properly? 8 Are in-text citations consistently and properly formatted in APA? Was each source cited at least once in the paper? 6 Are sources properly formatted in APA? Are there 10 sources, 5 of which are primary sources?

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RESULT PHOTOS : I am group 4 gram –

group1 is gram + (other group result) BSC 2011L Lab Report Guidelines
General Guidelines
The lab report must be in APA format:

typed
double spaced
12 pt font, Times New Roman
1 inch margins
pages numbered on the top right, running header on the top left
titled sections
untitled hypothesis
Quotes are NOT allowed. Everything must be properly paraphrased.
No website references as sources. No exceptions.
Everything must be properly cited. It is considered plagiarism if it is not.
Paper must be in third person, past tense
o Note: Never write statements like the following: “My lab report is
about…”, “My hypothesis is…”, or any version of this type of statement.
For more information on how to cite in APA, you may visit
https://owl.purdue.edu/owl/research_and_citation/apa_style/apa_style_introduction.html
The lab report will be composed of seven sections. Each section will have a heading
immediately followed by the text, figures or graphs. The order of the sections is: cover/title
page, abstract, introduction, methods, results, discussion and literature cited.
Cover Page
You must include a cover page with your name, panther ID, BSC2011L, your section,
TA name, date, and title of your paper.
Create a title that briefly conveys to the reader the purpose of the paper. The title of
your report must be informative. Many readers scan journal article titles and the
decision whether or not to pursue an article is based on the information in the title.
Generally, this information includes primary factor(s) manipulated or studied,
outcome of manipulation (the response or effects) and organism studied, if relevant.
Abstract
The abstract should be a quick but comprehensive summary of the entire report. It
serves to help readers determine how relevant the report is to their own interests.
This section should only be one paragraph and should include what was done, the
reasoning behind it, the results and the conclusions. It must highlight only the most
important elements of each major sections of the report (Introduction, Methods,
Results, and Discussion). Only the most important aspects of the report should make
it to the abstract.
This section should be between 200 and 250 words in length and should contain a
clear summary of what was demonstrated, how each part of the lab was carried out
and how conclusions were reached. It should also be on its own page.
Introduction
This section contains the basic background information for the lab report. Be sure to
comment on what is the significance of this study and its relation to the larger field.
Give an example of why the study is significant. After reading the introduction, the
reader should have a good idea of the general purpose of the experiment and the
importance of the problem being addressed. Here, you will also introduce the
purpose of your experiment. You should also be including your hypothesis and
predictions in this section – which should be based in the introduction (without
saying “My predictions are…” or “My hypothesis is…”).
This section should be about 2 pages long and smoothly flow from one topic to the
next. All of your background information should be presented here, so the majority
of your citations should be found here. You must include 10 sources, 5 of which are
primary sources. Remember: do NOT quote anything and make sure you are
properly paraphrasing – otherwise, you are PLAGIARIZING.
Methods
In this section, you must carefully outline the procedures and techniques used in a
way that a reader can replicate the experiment based on the information provided
here. This section should also include models or equipment used (this should not be
written like a laundry list of materials), source of chemicals (if relevant), numbers
and types of organisms used, including sex and strain sample sizes, number of times
experimental procedure was performed, and other pertinent factors. Keep in mind
the you used the methods in the lab manual, so you should be citing the lab manual.
Please note: This section must be a NARRATIVE – not bullet points or a numbered
list of steps. No credit will be given for a methods section written in a bulleted
format. Also, it must be past tense and in 3rd person – you should not be saying “first,
I did this…..” and so on.
Results
In this section, you will be presenting all of the observations and findings from the
experiment. It is crucial that the outcomes of your experiments are carefully
organized and clearly presented, and you must do so using tables, graphs and
pictures wherever necessary. Each figure should have a title or number and brief
caption that explains the information being presented by the figure.
All graphs and tables should be your own work! Graphs and tables should not be
identical to anyone else’s in the lab, including your lab partner’s.
Please note: You should NOT be interpreting the results in this section!! This section
focuses on what you observed quantitatively and qualitatively throughout the
collection of data. Any and all analysis belongs in the discussion section.
Discussion

In the discussion section, you should begin this section with restating the purpose
and hypothesis of your experiment. This section is where results are interpreted,
and conclusions are drawn. The significance and interpretation of the study should
be explained in this section as well. You should be referencing figures in the results
sections and explaining what these figures mean. Because you restated the
hypothesis before interpreting the results, you should be indicating whether or not
the results support your hypothesis – keyword: SUPPORT. Remember nothing is
EVER proven, so the results either support or refute your original
hypotheses/predictions.
In addition to explaining the results, you have to indicate the significance of your
results. What does this mean? How is it relevant or important? How can this
information be applied to the real world? If there were any errors made or any
limitations to the experimental design, this should also be included and in context
with the interpretation of the results. Please note: If you indicate any errors or
limitations, you must present SOLUTIONS to these issues, in the event that this
experiment will be replicated.
This section should be approximately 2-3 pages long and should be the longest part
of your lab report.
Literature Cited
This section includes the alphabetical listing of all sources cited in your paper. This
will primarily include research articles but may include review articles and texts as
well. The citations must be written using APA format. You need include at least 10
references, 5 of which must be primary sources. You may include your general
biology textbook and lab manual (don’t forget to cite the methods). No website
references or any encyclopedia references are allowed.
For each source in this section, YOU MUST HAVE IN-TEXT CITATIONS! Any intext citation in the body of the report is put at the end of a sentence and should be
done in APA format.
General APA citation Guidelines:
1. References should be listed in alphabetical order
2. If a particular reference takes more than one line, then all lines (except the first)
should be indented
3. Include ALL authors, never write “et al.,” when writing the full literature cited page
4. Remember to write “and” before the name of the last author, if the article or book
has multiple authors.
5. References should not be numbered nor bulleted.
6. Everything counts including but not limited to the placement of period, italics,
parentheses, etc.
7. If there are no in-text citations and no works cited section the report will be
considered plagiarized.
General Rules For the Lab Report:





If you are not present in lab you cannot turn-in a lab report for that lab.
You will turn in your lab report in its entirety. Turning in one or more components
or sections of the lab report after the due date and time will make the entire lab
report late.
This lab report must be your own work (no plagiarism). Content used from
references needs to be cited. All text and graphs should be original and not the
same as your partners. You cannot resubmit work previously done in another
class (per the plagiarism policy)
You will need to turn in only an electronic copy. Turnitin will be used to check for
plagiarism. 10 points per day will be deducted from your lab report until your
instructor has your report through Turnitin.
It is your responsibility to turn your lab report on time electronically to your
instructor, per their instructions. A late lab report will lose 10 points every day it is
late. It is your responsibility to know how to properly submit your report AND to
check to make sure it is submitted. Make sure you receive a confirmation email for
submitting on Turnitin.
BSC 2011L – General Biology II
Lab Report Rubric
Section
Cover Page
Points:
Does the cover page have an informative title?
Is all information (name, section, etc.) included?
2
Does the abstract reflect the entirety of the paper, including
methods, results and limitations?
Is the abstract on its own page?
Is the abstract length adequate (1 paragraph, 200-250
words)?
7
Is the topic clearly introduced and well researched?
Is the purpose of the experiment explained?
Is there a hypothesis?
Is the introduction at least 2 pages long?
20
Methods
Are the procedures correct?
Are all steps included?
Is this section a past-tense narrative (NOT in bullet points)?
6
Results
Are the observations clearly explained?
Are there relevant pictures/graphs/tables included, and if
so, is there a description under each?
Are results analyzed in this section? (they should NOT be).
10
Are the purpose and hypothesis restated?
Are the results well discussed?
Are real-world applications and significance of findings
included?
Are limitations and sources of error evaluated, and are
solutions provided for these?
25
Is paper in APA format?
Is the information clear and concise?
Does the paper flow and transition well, with no jumping
between topics?
10
Is there proper grammar, spelling and paraphrasing?
Are sentences structured properly?
8
Are in-text citations consistently and properly formatted in
APA?
Was each source cited at least once in the paper?
6
Are sources properly formatted in APA?
Are there 10 sources, 5 of which are primary sources?
6
Abstract
Introduction
Discussion
Organization/
Format
Language/
Grammar
In-Text Citations
Bibliography
del
7
end
t shift
(intet
CORE 17
EXERCISE 2
NOTES
5
NWY
THE SCIENTIFIC METHOD
What is called the scientific method is used by scientists to validate existing knowl-
edge, articulate cause-and-effect relationships, and test new ideas. There is no single
scientific method, no universal way of conducting research, but all science is based on
the general methodology: the steps that are required to produce reproducible results
and move an idea (a testable hypothesis) along the road to accepted conclusion, law,
or theory. The scientific method describes the steps needed to conduct meaning-
ful experiments with results that can be understood, reproduced, and validated by
others.
1
Observation a continuus pattern that you gather through senses
2. Research question inquiry based on observation
3 Review the literature compilation of research paper
testable y
4. Construct a hypothesis canid explanation for what you observed.
5 Make predictions showilotionphie bow dependents independent
6
Test with an experiment
Collect and analyze data
Ask a new question
or revise hypothesis
Interpret the data and
7 draw conclusions
No
Yes
Results support
the hypothesis?
8 Communicate results

Hayden-McNeil, LLC
Figure 2-1. The scientific method.
TASK 1: PRACTICAL APPLICATIONS OF SCIENTIFIC
METHODOLOGY: STERILIZATION, LIMITING BACTERIAL
COUNTS, AND BACTERIAL GROWTH
The population count (bacterial count), growth, and reproduction of bacterial patho-
gens may be regulated by:
Antibiotics (Greek: “anti,” against; “biotic,” life) are substances, including both natu-
ral and synthetic compounds, that act to kill or dramatically reduce the population of
bacteria. Antibiotics may kill other microbial life, including fungi, but do not harm
12
viruses. Bacteria may also produce compounds that ad as motos
bacteria. For example, the bacterium Streptomyces podem
antibiotic used for the treatment of active tuberculosis.plague tamaa
ber of other diseases caused by bacterial infection
Disinfectants are substances that kill bacteria and either simimate pratite
the population of bacteria (and/or fungi) on surfaces. Disinfecta
nent in controlling infection and lowering bacterial counts to saler
bleach can be used to disinfect work areas where bacteria srebranded
Antiseptics may kill bacteria, limit bacterial coumts by lowering the price
bacteria directly, and/or inhibit bacterial growth om ning suures as
not be chemical; ultraviolet (UV) light is a commonly used antiseptic for
sum
of medical and dental instruments. Chemical antisepties include bedragem
iodine, alcohol, boric acid, and a number of chlorides
Over time, the overuse and improper use of antibiotics has allowed for them
of antibiotic-resistant bacterial strains Evolution allows gemeentom image
isms, including bacteria. In many cases, antibioties de natal amet
of organisms, If, for example they kill 99.9% of the bactena, the hacemalonumis
reduced to a point where no disease is present and the immune sem candan
smaller lingering issues. However, the 1 percent of hacten that the
main
kill may still live because of some genetic advantage or difference thewhold As the
are the only bacteria left to reproduce the resistant genetic makes are usedom
to offspring who may then also exhibit resistance on a larger scale
As a result of this phenomenon, the number of antibiothee
is shrinking and the number of bacteria resistant to existing og
Some scientists are working on altering microbial genes to solve the podium
others search for natural or synthetic antibiotio fantiseptice
Aromatic botanical essential oils (including those of
commence and
thyme) may provide such an anti-resistance alternative love and the he
proven ettective in treating bacterial vaginosis and some stap
heal faster when treated with extracts and repors from
tom
Melaleuw alterfolie trees. Hucapus tres produce a pod
gingivitis and dental plaque
The de diffusion method tests species of bacteria pead over a de
agar Substances to he tested are placed on a discoffier that is the
the ager. The substances diffuse in the Agar and if they are
either limit or extinguish bacterial growth how and around the
This lab exercise explores the practical application of the
ing the antibacterial qualities of plant esential oils by testing them on gram
and gram negative hacerta
Ara das er in groups assigned define the following in terms in the
method and apply it to your experiment
gen
XERCISE 2
OTES
1

Jun
2
3
4
001
5a. Predictions on the effect of test compounds on bacterial growth
Disc Compound
Prediction
Cinamin ail
Eucalyptus
Tea tree al
Almond Oil
5 repermintoil
Penacilln
Null be nothing No substance so
6. Experiment, Data Collection, and Analysis
6a. Experiment
Define experiment:
Precedure
Scientific experiment that manipulates a variable to
nothing
test hypother’s
Independent variable for your experiment:
Dependent variable for your experiment:
Control variables for your experiment:
‘con
Experimental group for
your experiment:
Control
group for your experiment:
ving 7 Experimental group for your experiment:
eocom
6b. Data CollectionObservations on the effect of essential oils on
bacterial growth
r
Zone of Inhibition (mm)
mL
Compound
Your Group
Values
Antibacterial
Activity*
Average of Your
Group Values
radius
None
None
28
172
None
Plate 1 Plate 2
(in amin oil
Eunluptw oil
tea tree oil
28 28
Almond
rmint
In
Use the following criteria
6 mm = not antibacterial
>6 to 16 mm = slightly antibacterial
>16 mm = highly antibacterial
+
None
mene oma
None

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