University of Auckland Molar Concentration Lab Report I will upload the paper that you need to complete all information is written in the attachment please

University of Auckland Molar Concentration Lab Report I will upload the paper that you need to complete all information is written in the attachment please try doing it as accurate as you can if. there is any question contact me CHEM 151
Online Assignment 2 v5 | 1
151 Online Assignment 2 Version 5
Your name: _________________________
Your section: ____________________________
GRADE ____ /25 p
This assignment is due before 11 pm on Friday April 3rd, 2020. The deadline is absolute. A zero will automatically apply to a late
submission. You are being given more than sufficient time to complete this assignment, so please, no excuses.
All work must be well written and organized. Do not forget to title appropriately all graphs and correctly label all axes.
Assume you are working for a research lab studying the possible connection between consumption of FD&C food dyes and
various attention deficit issues in children. As part of this study, you must quantify the FD&C food dyes in various packaged
drinks typically consumed by children. One kiwi-lime-flavored drink lists two FD&C food dyes, Yellow #5 and Blue #1. Your
immediate task is to quantify these dyes in the drink, as the manufacturer will not release this information.
It is known that FD&C food dye Yellow #5 (MM = 534.37 g/mol) has a λmax = 426 nm, while Blue #1 (MM = 792.86 g/mol) has a
λmax of 630 nm. You sample 3.00 mL of the kiwi-lime-flavored drink into a standard polystyrene (b = 1.00 cm) cuvette and take
an initial absorbance vs. wavelength (A vs. λ) absorption spectrum with a spectrophotometer identical to that of the CHEM 151
labs and following a procedure analogous to that specified in the CHEM 151 videos. The absorbance exceeds 1.00, so you dilute
the kiwi-lime-flavored drink by transferring 1800 µL of the drink into a 2-dram vial and adding 2.20 mL of NANOpure water. This
diluted sample gives an absorption spectrum that clearly shows two distinct peaks, one with λmax = 426 nm, the other with λmax
= 630 nm. You record the absorbance values:
Absorbance vs. Wavelength (A vs. λ) Absorption Spectrum Peaks
for the Diluted Kiwi-Lime-Flavored Drink
Peak
λmax
Absorbance, A
1
426 nm
0.481
2
630 nm
0.307
1. Preparation Tables (9 p). Working with a stock solution of FD&C food dye Yellow #5 (3.09 x 10-5 M)
and another stock for FD&C food dye Blue #1 (5.97 x 10-6 M), you build two dilution sets using
NANOpure water as diluent. Complete the following tables giving an example calculation.
FD&C Food Dye Yellow #5 Dilution Set Preparation
Volume of Stock (µL)
Volume of Diluent (µL)
3000
0
0.913
2225
775
0.692
1500
1500
0.466
775
2225
0.241
50
2950
0.015
151 OA 2 v5 MY © 2020 3-26-20 Chem Think Technical Press CBC UofA
Diluted Concentration (M)
A at λmax = 426 nm
CHEM 151
Online Assignment 2 v5 | 2
FD&C Food Dye Blue #1 Dilution Set Preparation
Volume of Stock (µL)
Volume of Diluent (µL)
3000
0
0.782
2250
750
0.594
1500
1500
0.391
750
2250
0.191
100
2900
0.026
151 OA 2 v5 MY © 2020 3-26-20 Chem Think Technical Press CBC UofA
Diluted Concentration (M)
A at λmax = 630 nm
CHEM 151
Online Assignment 2 v5 | 3
2. Molar Absorptivity (8 p). Determine the molar absorptivity, ε, for the two dyes. Paste in all graphs.
Include the linear trendline equation(s) and R2 value(s). Clearly state the determined ε for each dye.
151 OA 2 v5 MY © 2020 3-26-20 Chem Think Technical Press CBC UofA
CHEM 151
Online Assignment 2 v5 | 4
3. Mass of Dye per Volume (8 p). Now calculate the mass in grams (g) of dye per milliliter (mL) of kiwilime-flavored drink. If a child consumes an average of 0.55 L of this drink per day, what is the weekly
exposure of each dye from this source in milligrams (mg)? To receive credit, you must present, in a highly
organized fashion, all the calculations.
151 OA 2 v5 MY © 2020 3-26-20 Chem Think Technical Press CBC UofA

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