University of Southern California Macroscopic Observation of Microorganisms Lab I will provide all the material necessary to do it. It shouldn’t take long

University of Southern California Macroscopic Observation of Microorganisms Lab I will provide all the material necessary to do it. It shouldn’t take long its very easy and the teacher will be able to help if needed, just tell me the question you have an I will ask it. The first attachment is for chapter 7 and the last attachment is for chapter 8. Name __________________________________
TA_____________________________________
Lab Day/Time____________________________
Laboratory Report (18 pts)
Microscope # ________
I. Macroscopic Observation of Microorganisms
Approx. number of organisms ___________ Number of
Colony Description
Colony Type I
organisms ___________
Colony Type II
Color
Size (diameter in mm)
Shape
Texture
Margin or Edge
Elevation
Questions
1. What is a colony?
2. Why is it important to characterize each colony type?
Chapter 7 – Microbes, Microscopy and the Compound Microscope
77
II. Microscopic Observation of Microorganisms
1. Sketch the cellular morphologies of the organisms observed on the commerciallyprepared slides.
a. Mixed bacterial smear
10
40
b. Gram-positive
40
100
c. Gram-negative
40
100
Chapter 7 – Microbes, Microscopy and the Compound Microscope
78
100
Questions
2. Why must care be taken to not substitute another oil for the immersion oil when making
microscopic observations?
3. How can the amount of light that passes through the slide to the ocular lens be
regulated?
4. How can the resolution of the microscope be improved?
Chapter 7 – Microbes, Microscopy and the Compound Microscope
79
Chapters 7 and 8
These photos replace the in-person microscopic views you would have
observed in lab. Complete your lab report like normal using these slides.
(Note that you don’t have multiple magnifications for the bacteria or multiple
scopes for the fungi, so you will need to leave some circles blank in the lab
report.
Chapter 7
Microbes, Microscopy, and the Compound Microscope
I. Macroscopic Observation of Microorganisms
https://everythingmicro.blogspot.com/2018/06/basicbacterial-morphology-bacteria-and.html
II. Microscopic Observations of Microorganisms
Mixed Bacterial Smear

II. Microscopic Observations of Microorganisms
Gram-positive Bacteria
https://www.researchgate.net/post/How_can_I_identify_these
_three_types_of_gram_positive_bacilli_bacteria
II. Microscopic Observations of Microorganisms
Gram-negative Bacteria
https://www.123rf.com/photo_38484872_gram-staining-gramnegative-bacilli-.html
Chapter 8
Eukaryotes: Protists and Filamentous Fungi
A. Protists
Euglena sp.
https://www.thoughtco.com/about-euglena-cells-4099133
A. Protists
Paramecium caudatum
https://commons.wikimedia.org/wiki/File:Grupo_de_Parameci
um_caudatum.jpg
A. Protists
Amoeba proteus
https://fineartamerica.com/featured/amoeba-proteus-powerand-syred.html
A. Protists
Mixed Pond Sample

A. Protists
Diatoms

A. Protists
Dinoflagellates
http://nathistoc.bio.uci.edu/Dinoflagellates/index.htm
B. Fungi
Rhizopus
http://twojdoktor.info/
B. Fungi
Aspergillus

The fallacy of Aspergillus

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B. Fungi
Penicillium

B. Fungi
Coprinus
https://www.picfair.com/pics/01688396-coprinus-mushroomsection-stained

Name __________________________________
TA_____________________________________
Lab Day/Time____________________________
Laboratory Report (20 pts)
Microscope # ________
A. Protists
1. Make colored sketches of the single, known cultures of protists.
– Indicate the name of the protist AND its supergroup.
a. Identify and label any morphological features of the protists observed in the known
cultures.
8 – Eukaryotes: Protists and Filamentous Fungi
91
2. Make colored sketches of the protists in the mixed samples. Indicate the sample below your
sketches.
a. Mixed pond sample (
):
b. Mixed marine samples (
):
c. Identify and label any morphological features of the protists observed in the pond
and marine cultures.
Chapter 8 – Eukaryotes: Protists and Filamentous Fungi
92
Questions
1. What morphological features were most commonly observed? Describe their apparent
functions.
2. On the basis of observations made, what seems to be the function of cilia?
3. What is the color of the pigmented substances within the cytoplasm of the protists?
Explain the occurrence of these pigmented substances?
B. Fungi
1. Make sketches of your mold cultures and identify (label) all structures.
a.
Dissecting scope
Compound scope ____
8 – Eukaryotes: Protists and Filamentous Fungi
93
b.
Dissecting scope
Compound scope ____
Dissecting scope
Compound scope ____
c.
d.
Cross section (c.s.)
Compound scope (10
Chapter 8 – Eukaryotes: Protists and Filamentous Fungi
94
Longitudinal section (l.s.)
Compound scope (10
Gills from longitudinal section
Compound scope (40 )
Questions
1. The body form of most fungi, including
, is a mycelium composed of
form well adapted to the fungus mode of nutrition?
2. Are the spores of
are they more like those of
produced in a sporangium as are those of
? Explain.
, or
3. Many mycologists (scientists who study fungi) use the term “mitospore” for the asexual
spores of fungi. Why do you think they use that term (i.e., what does the term suggest)?
4. What is the possible evolutionary advantage to the fungus for the following:
a.
makes and secretes an antibiotic.
b. Ergot fungus (parasitizes rye grain) produces a chemical that is toxic to animals.
8 – Eukaryotes: Protists and Filamentous Fungi
95

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